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Anti-5-Methylcytosine Antibody (33D3)

Monoclonal antibody to detect 5-Methylcytosine
Catalog #: A1294

Product Details

Cat # +Size A1294-50
Size 50 µg
Antibody Target 5-mc
Alternate Name 5-mc, 5mc
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG1, Lambda
Immunogen Modified ribonucleoside, specific for the presence of a methyl group on carbon 5 of the pyrimidine ring
Appearance Colorless liquid
Formulation Phosphate Buffer 10mM - NaCl 0.15 M - pH 7.4
Purification Affinity purified
Species Reactivity Human, Mouse, Rat
Application WB, ELISA, MeDIP/ChIP, IF, , IHC, FC
Application & Usage WB; 1:250, ELISA: 1:10000, MeDIP/ChIP: 1-2 µg per IP, IF: 1:500, IHC Perform heat mediated antigen retrieval before commencing with IHC staining protocol, ICC; 0.5-5 µg/ml, FC; 10µl of working dilution to label 10 x 106 cells in 100µl
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


5-methylcytosine (5-mC) is a modified base present in nucleic acids of plants and vertebrates. DNA methylation is a post-replicative process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Carcinogenesis is associated with alterations of the DNA methylation pattern: a global hypomethylation is often detected in tumor tissues when compared to their normal counterpart. Simultaneously local hypermethylation sites are observed

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