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Anti-5-Hydroxymethylcytosine Antibody (4D9)

Monoclonal antibody to detect 5-Hydroxymethylcytosine
Catalog #: A1295

In stock

$315.00

Product Details

Cat # +Size A1295-50
Size 50 µg
Antibody Target 5-hmc
Alternate Name 5-hmc, 5hmc
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG1
Immunogen 5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven
Appearance Colorless liquid
Formulation Phosphate Buffer 10mM - NaCl 0.15 M - pH 7.5
Purification Affinity purified
Species Reactivity Human, Mouse, Rat
Application ELISA, hMeDIP IF, Dot Blot
Positive Control Dot Blot, ELISA
Application & Usage ELISA: 1:1000, hMeDIP: 1-3 µg per IP, IF: 1:500, Dot Blotting: 1:2,000
Handling The antibody solution should be gently mixed before use
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.


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