Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
Overview
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Product name
Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent
See all Annexin V/ANXA5 reagents -
Tested applications
Suitable for: FM, Flow Cytmore details -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 1013 Annexin V-Cy5 Reagent.
Annexin V/ANXA5-Cy5 Apoptosis Detection Reagent ab14147 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
This Annexin V/ANXA5-Cy5 reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-Cy5 reagent is also available as part of Annexin V/ANXA5-Cy5 apoptosis kit ab14150.
For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA -
Concentration information loading...
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Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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Related Products
- Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
- 10X Annexin V/ANXA5 Binding Buffer (ab14084)
- Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
- Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
- Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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FM |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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Notes |
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FM
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab14147 has been referenced in 2 publications.
- Guo X et al. Global characterization of T cells in non-small-cell lung cancer by single-cell sequencing. Nat Med 24:978-985 (2018). PubMed: 29942094
- Wu KW et al. Neurovascular Interaction Promotes the Morphological and Functional Maturation of Cortical Neurons. Front Cell Neurosci 11:290 (2017). PubMed: 28966577