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Annexin V-Cy5 Reagent

based on 16 citations in multiple journalsAnnexin V-Cy5 Reagent164.6 5
Cy5 conjugated Annexin V for detection of apoptosis through fluorescence microscopy or by flow cytometry
Catalog #: 1013
SKU-Size Size Price Qty
1013-200 200 assays
1013-1000 1000 assays
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Product Details

Highlights • Detection method- flow cytometry (Ex/Em = 649 /670 nm) using Helium-Neon Laser and fluorescence microscopy
• Sample- Cell culture
• Simple, easy to use and convenient
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Annexin V-Cy5 is a bright fluorescent reagent for detecting of the early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca²ᶧ-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. Cy5 fluorescent dye produces an intense signal in the far-red region of the spectrum and therefore it is very useful for multiple labeling of cells with green and red colored fluorescent probes. Cy5 yields fluorescence with a λmax emission of 670 nm.

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Lukas Wisgrill, Influence of hemoadsorption during cardiopulmonary bypass on blood vesicle count and function. J Transl Med., May 2020;  32414386.
Scheffel, Matthew J. et al. (2016) Efficacy of Adoptive T-cell Therapy Is Improved by Treatment with the Antioxidant N-Acetyl Cysteine, Which Limits Activation-Induced T-cell Death, Cancer Res. 2016 Oct 15;76(20):6006-6016.
Liu et al., Adapting a Drug Screening Platform to Discover Associations of Molecular Targeted Radiosensitizers with Genomic Biomarkers. Mol. Cancer Res., Apr 2015; 13: 713 - 720.
Alagesan et al., Combined MEK and PI3K Inhibition in a Mouse Model of Pancreatic Cancer. Clin. Cancer Res., Jan 2015; 21: 396 - 404.
Keerthivasan et al., Aberrant overexpression of CD14 on granulocytes sensitizes the innate immune response in mDia1 heterozygous del(5q) MDS.Blood, Jul 2014; 124: 780 - 790.
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