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Angiogenesis (Tube Formation) Assay

Catalog #: K905
$395.00

Product Details

Cat # +Size K905-50
Size 50 assays
Detection Method Light & Fluorescence microscope
Applications Screening inhibitors and stimulators of angiogenesis
Study of angiogenesis related signal transduction
Features & Benefits • Robust method to determine angiogenesis (in vitro) in less than 18 hrs
• Simple & convenient
• semi-quantitative tool for assessing angiogenesis
Kit Components • Extracellular Matrix Solution (2 vials)
• Wash Buffer
• Staining Dye Concentrate
• Inhibitor Control (Suramin)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Angiogenesis is the process of generating new blood vessels from the pre-existing vasculature. Angiogenesis is required for growth and development, wound healing, tissue granulation and formation of malignant tumors. The quick assessment of angiogenesis involves measurement of the ability of endothelial cells to form three-dimensional tube-like structures. BioVision’s Tube Formation Assay provides a robust method to determine angiogenesis (in vitro) in less than 18 hrs. This assay kit provides a simple, easy to perform, semi-quantitative tool for assessing angiogenesis.


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Is the ECM solution growth factor reduced?
Yes, the ECM solution is growth factor reduced.
May I know if you have any stability information regarding the Staining Dye- i.e. will the fluorescence dye fade away very quickly?
Since the dye does not get incorporated or covalently attach to any cellular components after they enter the cells, they may have a short retention time as some cell types may actively efflux the dye out of the cell. Therefore, we recommend observing the cells as soon as the staining is done as it may fade soon.
What type of endothelial cells was tested with the kit? Have you tested HDMECs?
We used EA.hy926 cells to optimize the kit. We have not tested HDMECs with this kit.