Product Details
| abID | ab123226 |
|---|---|
| Alternate Name | ANGPT-2 |
| Gene Symbol | ANGPT2 |
| Gene ID | 285 |
| Accession # | O15123 |
| Source | CHO cells |
| Appearance | Lyophilized powder |
| Physical Form Description | Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 8.0. |
| Molecular Weight | 60-70 kDa |
| Purity by SDS-PAGE | ≥95% |
| Endotoxin Level | < 0.1 ng/μg of protein (<1EU/μg). |
| Biological Activity | Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2 μg/ml. |
| Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliq |
| Amino Acid Sequence | DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQQNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE LQLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHIIQLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QHDLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTNGI YT |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha helix rich “coiled coil” N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0– 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.
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According to a MW calculator, the sequence provided on the web page (http://www.biovision.com/ang2humanrecombinant7056.html) is about 50kDa. This product is 6070kDa according to your website. Can you please confirm if there is a tag on this protein and if so what type of tag it is?
The calculated molecular weight of Recombinant Human ANG2 is 50.1 kDa. However in the description we do list that the recombinant Human ANG2 is a Cterminal histidinetagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDSPAGE under reducing conditions
