ANA Screen (Antinuclear Antibody) ELISA Kit

An ELISA kit for in vitro detection of IgG class antibodies to ANA in human serum samples

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4708 | abID:

Product Details

Cat # +Size E4708-100
Size 96 assays
Detection Method Absorbance-450 nm
Species Reactivity Human
Applications Detection of Antinuclear Antibody in human serum and plasma samples
Kit Components • Stop Solution
• Microwells coated with nuclear antigens
• Positive Control
• Sample Diluent
• Enzyme conjugate
• Negative Control
• TMB Substrate
• Calibrator
• Wash buffer (20X)
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Antinuclear antibodies (ANA) are frequently present in patients with systemic lupus erythematosus (SLE) and, less commonly, in other autoimmune diseases Rheumatoid arthritis, Collagen vascular diseases, chronic liver diseases and systemic sclerosis (scleroderma). ANA bind to several nuclear antigens including DsDNA, SSDNA, RNP, Sm, SSA and SSB. ANA frequency increases with age in apparently healthy people, especially women after the age of 45 years. ANA ELISA is widely used as a screening procedure for different autoimmune diseases. BioVision’s ANA Screen ELISA test system is an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG class antibodies to ANA in human serum or plasma. Diluted patient serum is added to the ELISA plate coated with purified nuclear antigens. ANA IgG specific antibody binds to the antigen present in the samples. The enzyme conjugate is added to bind to the antibody-antigen complex. After the addition of substrate, the plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

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