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AMP Colorimetric Assay Kit

To detect AMP in biological samples
Catalog #: K229
$485.00

Product Details

Cat # +Size K229-100
Size 100 assays
Kit Summary BioVision’s AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.
Detection Method Absorbance, OD 570 nm
Species Reactivity All
Applications • Study the regulation of AMPK by AMP
• Mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc.
• Measurement of AMP level in various cell or tissue types
Features & Benefits • Sensitive
• High-throughput adaptable
• It can measure AMP level lower than 10 µM in various sample types
• Easy to use
• Rapid
Kit Components • AMP Standard
• AMP Enzyme
• AMP Assay Buffer
• AMP Substrate Mix
• AMP Developer
• AMP Probe
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Adenosine Monophosphate (AMP), also known as 5’-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories. BioVision’s AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.


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