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Amikacin ELISA Kit

A competitive enzyme immunoassay for the quantitative analysis of AMK concentration in various biological samples including urine, serum and plasma.

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to
www.P65Warnings.ca.gov.
Catalog #: E4912
$695.00

Product Details

Cat # +Size E4912-100
Size 96 assays
Kit Summary BioVision’s Amikacin ELISA Kit is a competitive enzyme immunoassay for quantitative analysis of AMK concentration in various biological samples including urine, serum and plasma.
Detection Method Absorbance at 450 nm
Species Reactivity Universal
Applications In vitro quantitative determination of AMK in serum, plasma and urine samples.
Features & Benefits ● The limit of detection of the kit is 0.1 ng/ml.
● The kit is simple, easy and reliable to determine the AMK concentration (0.3 – 24.3 ng/ml) in less than 90 minutes.
Kit Components ● Pre-coated 96 Well Strip Plate
● Conjugate Buffer
● Plate Sealers
● AMK Standard
● HRP Conjugate
● Anti-AMK Mouse Antibody
● TMB Substrate
● Stop Solution
● Wash Buffer (10X)
● Sample Diluent
● Standard Buffer
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Amikacin (AMK) is an aminoglycoside antibiotic mainly used to treat bacterial infections caused by gram negative bacteria such as Pseudomonas, Acinetobacter, Enterobacter, E. coli and Klebsiella and gram positive bacteria such as Staphylococcus and Nocardia. It is a protein synthesis inhibitor and kills bacteria by blocking the function of the 30S ribosomal subunit. AMK is on list of essential medicines of World Health Organization AMK is most commonly used for treating meningitis, pneumonia, sepsis, joint, intra-abdominal and urinary tract infections. However, AMK can cause serious neurotoxicity and renal toxicity issues. The side effects include paralysis, hearing loss, kidney failure, balance problems etc. BioVision’s Amikacin ELISA Kit is a competitive enzyme immunoassay for quantitative analysis of AMK concentration in various biological samples including urine, serum and plasma. In this assay, the AMK conjugate is pre-coated on the 96-well plate. AMK in the sample and the AMK conjugate on the plate compete for binding to the anti-AMK antibody. After the addition of the HRP conjugate that binds to the captured AMK antibody, TMB substrate is added and a color signal is developed, which is measured at 450 nm. The OD value of the sample is inversely proportional to the AMK concentration in the sample. This value is then compared to the AMK standard curve and the AMK concentration is obtained. The kit is simple, easy and reliable to determine the AMK concentration (0.3 – 24.3 ng/ml) in less than 90 minutes. The limit of detection of the kit is 0.1 ng/ml.


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