Alpha Ketoglutarate (alpha KG) Assay Kit (ab83431)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.01 nmol/well
Overview
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Product name
Alpha Ketoglutarate (alpha KG) Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
= 0.01 nmol/well -
Range
0.01 nmol/well - 10 nmol/well -
Assay time
0h 30m -
Product overview
Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.
In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe to a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product.
Alpha Ketoglutarate assay protocol summary:
- add reaction mix to standard and sample wells
- incubate for 30 min
- analyze with a microplate reader
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K677 Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit. K677-100 is the same size as the 100 test size of ab83431.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests a-KG Assay Buffer 1 x 25ml Converter Enzyme II 1 vial α-KG Standard 1 vial Development Enzyme Mix I 1 vial OxiRed Probe 1 x 200µl -
Research areas
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Relevance
Alpha ketoglutarate (Alpha-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing Alpha-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. Alpha-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. -
Alternative names
- Alpha KG
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab83431 has been referenced in 34 publications.
- Liu H et al. RUNX3-mediated circDYRK1A inhibits glutamine metabolism in gastric cancer by up-regulating microRNA-889-3p-dependent FBXO4. J Transl Med 20:120 (2022). PubMed: 35272674
- Parmana IMA et al. Myocardial Protecting Role of Glutamine in Patients with Low Ejection Fraction Undergoing Elective On-Pump Coronary Artery Bypass Graft Surgery. Vasc Health Risk Manag 18:219-231 (2022). PubMed: 35411147
- Cao S et al. Glutamine is essential for overcoming the immunosuppressive microenvironment in malignant salivary gland tumors. Theranostics 12:6038-6056 (2022). PubMed: 35966597
- Han L et al. MicroRNA 101 Attenuated NSCLC Proliferation through IDH2/HIFα Axis Suppression in the Warburg Effect. Oxid Med Cell Longev 2022:4938811 (2022). PubMed: 36304962
- Choudhury D et al. Inhibition of glutaminolysis restores mitochondrial function in senescent stem cells. Cell Rep 41:111744 (2022). PubMed: 36450260