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Alpha Crosslaps (αCTx) (Human) ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of α-CrossLaps concentrations in serum, plasma and other biological fluids.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4797

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications A Competitive ELISA kit for in vitro quantitative determination of α-CrossLaps concentrations in serum, plasma and other biological fluids.
Features & Benefits • Detection Range: 93.75-6000 pg/mL
• Specificity: No Significant cross-reactivity or interference between Human α-CTx and analogues was observed.
• Sensitivity: 56.25 pg/mL
• Precision:Coefficient of variation is < 10%.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


CrossLaps (CTx) peptide [Glu-Lys-Ala-His-Asp-Gly-Gly-Arg], a part of the C-telopeptide of the alpha 1-chain of type I collagen of bone, is developed as a biochemical marker of bone turnover. The Alpha CrossLaps is intended for use as an indicator of degradation of non-isomerized bone collagen and may be used as an aid in the identification of skeletal metastases in patients with breast and prostate cancer. BioVision’s Alpha Crosslaps (αCTx) (Human) ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with αCTx. During the reaction, αCTx in the samples or standard competes with αCTx coated on the plate for binding to the anti- αCTx antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of αCTx. The concentration of αCTx in the samples can be calculated by comparing the OD of the samples to the standard curve.

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