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Alpha 1-Antichymotrypsin ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Alpha 1-Antichymotrypsin in human stool samples.
Catalog #: E4702
$565.00

Product Details

Cat # +Size E4702-100
Size 96 assays
Kit Summary Detect Alpha 1-Antichymotrypsin in human stool samples.
Detection Method Absorbance-450 nm
Species Reactivity Human
Features & Benefits Detection of Alpha 1-Antichymotrypsin in human stool samples.
Kit Components • Sample Diluent: 3 bottles
• Alpha 1-Antichymotrypsin Controls : 2 vials
• Anti-ACT Enzyme Conjugate: 1 vial (ready to use)
• TMB Substrate: 1 bottle (ready to use)
• 20X Wash concentrate: 1 bottle
• Incubation Buffer: 1 bottle (ready to use)
• Stop Solution: 1 bottle (ready to use)
• Alpha 1-Antichymotrypsin Standard: 8 vials (ready to use)
• Microwell plate coated with anti-ACT Polyclonal Ab
Storage Conditions 2-8°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

ACT functions as a specific inhibitor of chymotrypsin-like serine proteases. BioVision Alpha 1-Antichymotrypsin (ACT) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-ACT polyclonal antibody, along with the incubation buffer. After a simple washing step, an anti-ACT enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of ACT in the samples. A standard curve is generated relating color intensity to the concentration of ACT.


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