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Alkaline phosphatase, mouse recombinant

A hydrolase enzyme responsible for removing phosphate groups
Catalog #: P1098

In stock

$125.00

Product Details

Cat # +Size P1098-10
Size 10 μg
Alternate Name Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS
Gene Symbol Aplp
Gene ID 11647
Accession # P09242
Source Insect cell (Baculovirus)
Appearance Liquid
Physical Form Description In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Weight 54.5 kDa
Purity by SDS-PAGE ≥95%
Endotoxin Level < 1 EU/μg
Biological Activity Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Amino Acid Sequence VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GL
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Alpl, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. A proposed function of this form of the enzyme is matrix mineralization. Recombinant mouse Alpl, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.


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