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Product Details
| Cat # +Size | K2117-100 |
|---|---|
| Size | 100 assays |
| Kit Summary | BioVision’s Alkaline Phosphatase Activity Assay Kit utilizes AquaSpark™, a water-soluble chemiluminescent dioxetane ALP substrate that enables the ultra-sensitive detection of ALP activity in various sample types. |
| Detection Method | Luminescence |
| Applications | ● Detection of alkaline phosphate activity in induced pluripotent and mesenchymal stem cells ● Conversion of ELISAs with ALP-conjugated secondary detection to chemiluminescence ● Estimation of alkaline phosphatase activity in various biological samples |
| Features & Benefits | The kit has a limit of detection of 0.005 μU/well of ALP activity in samples. |
| Kit Components | ● ALP Assay Buffer ● AquaSpark™ ALP Substrate ● ALP Lysis Buffer ● ALP Positive Control |
| Storage Conditions | -20ºC |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Alkaline phosphatase(s) (ALP, EC 3.1.3.1) are a family of enzymes that are expressed in virtually all living organisms. The ALP superfamily has extremely broad substrate specificity, hydrolyzing many different chemically distinct phosphate esters to liberate inorganic phosphate. In humans, there are four ALP isozyme types: intestinal, placental, placental-like and tissue non-specific enzymes. The tissue non-specific form, in particular is highly expressed in pluripotent and multipotent stem cells such as bone-marrow derived mesenchymal stems cells. ALP is also a popular enzyme conjugate used to generate signal output in enzymatic immunoassays. BioVision’s Alkaline Phosphatase Activity Assay Kit utilizes AquaSpark™, a water-soluble chemiluminescent dioxetane ALP substrate that enables the ultra-sensitive detection of ALP activity in various sample types. In this assay, the enzymatic cleavage of the phosphate ester moiety in the AquaSpark™ substrate yields an intermediate product that undergoes rapid self-immolative chemiexcitation, emitting bright green light (λem = 510 nm). The luminescent substrate has a high quantum yield in aqueous solutions and does not require any surfactants, enhancers or organic solvents to achieve maximum light emission. Because the AquaSpark™ luminescent probe is non-cytotoxic, the kit can also be used to identify pluripotent/multipotent stem cells or monitor osteogenic differentiation of mesenchymal stem cells without requiring lysis or fixation. The kit has a limit of detection of 0.005 μU/well of ALP activity in samples and can also be used for chemiluminescent detection in ELISAs with ALP-conjugated secondary antibodies.
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