Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

New

Alkaline Phosphatase Activity Assay Kit (Luminometric)

A 96-well plate based luminometric assay for the detection of alkaline phosphatase activity in various biological samples
Catalog #: K2117
$495.00

Product Details

Cat # +Size K2117-100
Size 100 assays
Kit Summary BioVision’s Alkaline Phosphatase Activity Assay Kit utilizes AquaSpark™, a water-soluble chemiluminescent dioxetane ALP substrate that enables the ultra-sensitive detection of ALP activity in various sample types.
Detection Method Luminescence
Applications ● Detection of alkaline phosphate activity in induced pluripotent and mesenchymal stem cells
● Conversion of ELISAs with ALP-conjugated secondary detection to chemiluminescence
● Estimation of alkaline phosphatase activity in various biological samples
Features & Benefits The kit has a limit of detection of 0.005 μU/well of ALP activity in samples.
Kit Components ● ALP Assay Buffer
● AquaSpark™ ALP Substrate
● ALP Lysis Buffer
● ALP Positive Control
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Alkaline phosphatase(s) (ALP, EC 3.1.3.1) are a family of enzymes that are expressed in virtually all living organisms. The ALP superfamily has extremely broad substrate specificity, hydrolyzing many different chemically distinct phosphate esters to liberate inorganic phosphate. In humans, there are four ALP isozyme types: intestinal, placental, placental-like and tissue non-specific enzymes. The tissue non-specific form, in particular is highly expressed in pluripotent and multipotent stem cells such as bone-marrow derived mesenchymal stems cells. ALP is also a popular enzyme conjugate used to generate signal output in enzymatic immunoassays. BioVision’s Alkaline Phosphatase Activity Assay Kit utilizes AquaSpark™, a water-soluble chemiluminescent dioxetane ALP substrate that enables the ultra-sensitive detection of ALP activity in various sample types. In this assay, the enzymatic cleavage of the phosphate ester moiety in the AquaSpark™ substrate yields an intermediate product that undergoes rapid self-immolative chemiexcitation, emitting bright green light (λem = 510 nm). The luminescent substrate has a high quantum yield in aqueous solutions and does not require any surfactants, enhancers or organic solvents to achieve maximum light emission. Because the AquaSpark™ luminescent probe is non-cytotoxic, the kit can also be used to identify pluripotent/multipotent stem cells or monitor osteogenic differentiation of mesenchymal stem cells without requiring lysis or fixation. The kit has a limit of detection of 0.005 μU/well of ALP activity in samples and can also be used for chemiluminescent detection in ELISAs with ALP-conjugated secondary antibodies.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.