Aldolase Activity Assay Kit (Colorimetric) (ab196994)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 100 µU
Overview
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Product name
Aldolase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Sensitivity
< 100 µU -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Aldolase Activity Assay (Colorimetric) (ab196994), aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.
This assay kit is simple, sensitive and high-throughput adaptable and it can be used to measure aldolase activity in various cells and tissues as well as to analyze glycolysis and glucogenesis pathways.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K665 Aldolase Activity Colorimetric Assay Kit. K665-100 is the same size as the 100 test size of ab196994.
Aldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone.
There are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy & hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase.
Accurate detection of aldolase activity is valuable for diagnostic and mechanistic studies.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PGK Developer 1 vial Aldolase Substrate 1 vial Assay Buffer IV 1 x 25ml Developer Solution III 1 vial NADH Standard I 1 vial Aldolase Positive Control 1 vial -
Research areas
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Function
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. -
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Involvement in disease
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Alternative names
- ALDA
- Aldo1
- ALDOA
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab196994 has been referenced in 13 publications.
- Bonet JB et al. Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners. Int J Environ Res Public Health 19:N/A (2022). PubMed: 35010758
- Zhu L et al. Myostatin Knockout Limits Exercise-Induced Reduction in Bovine Erythrocyte Oxidative Stress by Enhancing the Efficiency of the Pentose Phosphate Pathway. Animals (Basel) 12:N/A (2022). PubMed: 35405915
- Zhu Z et al. Reducing the Glucose Level in Pre-treatment Solution Improves Post-thaw Boar Sperm Quality. Front Vet Sci 9:856536 (2022). PubMed: 35433908
- Wan N et al. Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome. Nat Methods 19:854-864 (2022). PubMed: 35761067
- Haythorne E et al. Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells. Nat Commun 13:6754 (2022). PubMed: 36376280