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Aldehyde Dehydrogenase 2 Inhibitor Screening Kit (Fluorometric)

For screening, studying and characterizing potential inhibitors of ALDH2
Catalog #: K2011
$495.00

Product Details

Cat # +Size K2011-100
Size 100 assays
Kit Summary BioVision’s Aldehyde Dehydrogenase 2 Inhibitor Screening Kit provides a quick and sensitive way of screening, studying and characterizing potential inhibitors of ALDH2. In this assay, acetaldehyde is oxidized by ALDH2 to form NADH, which then couples with PicoProbe™ to generate a fluorescence signal measured at Ex/Em = 535/587 nm. The percentage inhibition can be determined by comparing the activity of ALDH2 treated with test inhibitor(s) vs untreated control.
Detection Method Fluorescence at Ex/Em = 535/587 nm
Species Reactivity Human
Applications • Screening or characterizing ALDH2 inhibitors
Features & Benefits • A quick and sensitive way of screening, studying and characterizing potential inhibitors of ALDH2
Kit Components • ALDH2 Assay Buffer
• Recombinant ALDH2
• ALDH2 Substrate
• ALDH2 Substrate Mix
• PicoProbeTM
• Inhibitor (Disulfiram, 2 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Aldehyde Dehydrogenase 2 (ALDH2), mitochondrial is a member of the ALDH superfamily that participates in multiple metabolic pathways including the oxidation of toxic biogenic and environmental aldehydes. ALDH2 is a key enzyme located in the mitochondrial matrix. It plays an important role in ethanol metabolism by converting acetaldehyde, a carcinogenic metabolite of ethanol to acetate. Imbalances of ALDH2 have been linked to both alcoholism and alcohol sensitivity in people. Reduced ALDH2 activity has been strongly correlated with reduced alcohol consumption and reduced incidences of alcoholism, which led to the development of selective ALDH2 inhibitors as antidipsotropic or alcohol aversive agents for treating alcoholism. Disulfiram, an alcohol-aversive drug inhibiting ALDH2 has been approved by the FDA and is used clinically for over 40 years for treating alcohol dependency. However, the drug is a non-specific alkylating agent leading to many adverse side effects and drug-drug interactions. Thus, novel and specific inhibitors of ALDH2 free of the negative side effects could be used in aversion therapy. BioVision’s Aldehyde Dehydrogenase 2 Inhibitor Screening Kit provides a quick and sensitive way of screening, studying and characterizing potential inhibitors of ALDH2. In this assay, acetaldehyde is oxidized by ALDH2 to form NADH, which then couples with PicoProbe™ to generate a fluorescence signal measured at Ex/Em = 535/587 nm. The percentage inhibition can be determined by comparing the activity of ALDH2 treated with test inhibitor(s) vs untreated control.


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