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ALCAM/CD166 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of ALCAM

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5029
$750.00

Product Details

Cat # +Size E5029-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of ALCAM in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Sensitivity: 37.5 pg/ml
● Highly sensitive and specific
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Detection range: 62.5-4000 pg/ml
● Recovery Rate: 90-103% for serum, 87-103% for EDTA plasma and 88-100% for heparin plasma
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (4 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

ALCAM/CD166 antigen is a 100-105 kDa type I transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 is involved in neurite extension by neurons via heterophilic and homophilic interactions. It is increased in various types of cancer. BioVision’s ALCAM/CD166 (Human) ELISA Kit is used for the quantitative detection of ALCAM in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ALCAM in the sample.


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