Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

AKT1 (Thr308) Antibody

Polyclonal Antibody to detect AKT1 in human, mouse, rat, bovine and xenopus samples.
Catalog #: 6742
$270.00

Product Details

Cat # +Size 6742-100
Size 100 μl
Antibody Target AKT1
Alternate Name PKB, RAC, Protein kinase B, Protein kinase B alpha, Short PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-309 amino acids from human AKT1.
Accession # P31749
Gene ID 207
Appearance Colorless liquid
Formulation 100 µl of antibody in PBS with 0.09% (W/V) sodium azide
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Species Reactivity Human. Predicted cross reactivity with Mouse, Rat, Bovine and Xenopus samples.
Application Western blot, IHC, FACS.
Positive Control Western blot: 293 cell line lysates and mouse liver tissue lysates, IHC: human breast carcinoma tissue , FACS: MDA-MB435 cells.
Application & Usage Western blot: ~1:1000, IHC: ~1:50-1:100, FACS: ~1:10-1:50.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.