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Product Details
| Cat # +Size | 6745-100 |
|---|---|
| Size | 100 μl |
| Antibody Target | AKT1 |
| Alternate Name | PKB, RAC, Protein kinase B, Protein kinase B alpha, Short PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase. |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the N-terminal region of human AKT1. |
| Accession # | P31749 |
| Gene ID | 207 |
| Appearance | Colorless liquid |
| Formulation | 100 µl of antibody in PBS with 0.09% (W/V) sodium azide |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Species Reactivity | Human. Predicted cross reactivity with mouse and Bovine samples. |
| Application | Western blot, IHC, FACS, IF. |
| Positive Control | Western blot: HeLa and 293 cell lysates, IHC: Human breast carcinoma, FACS: MDA-MB435 cells, IF: MDA-MB435 cells. |
| Application & Usage | Western blot: ~1:1000, IHC: ~1:50-1:100, FACS: ~1:10-1:50, IF: ~1:10-1:50. |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
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