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Akt1, Active

Protein involved in cellular survival pathways
Catalog #: 7701
SKU-Size Size Price Qty
7701-5 5 μg
$295.00
7701-100 100 μg
$3,950.00
More Sizes Get Quote

Product Details

Alternate Name Akt, RAC-alpha serine/threonine-protein kinase
Gene Symbol AKT1
Gene ID 207
Accession # P31749
Source Baculovirus (Sf9 insect cells)
Appearance Liquid
Physical Form Description Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight ~85.0 kDa
Purity by SDS-PAGE ≥90%
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

Akt1 or Protein Kinase B α(PKBα) is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt1 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2 (possibly ILK) (3). Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (4). In addition, the cyclin dependent kinase inhibitors are substrates of Akt which when phosphorylated relinquish their inhibitory influence on cell cycle progression. Akt also mediates many of the stimulatory effects of insulin on glucose metabolism through deactivation of glycogen synthase kinase, activation of phosphofructo-kinase, and modulation of glucose transporter activity. Akt1 has been shown to play a role in human cancers (4). It is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (5). Akt1 plays an important role in cancer cell survival and proliferation thereby contributing to cancer progression.


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Can we remove the GST tag. If so, how can we remove it?
Yes, you can remove the GST tag from the active enzyme by using thrombin. By using this method, you are removing the tag, but you still have GST tag floating in your solution. In order to avoid the presence of GST in your solution, you have to use a column, and pass the solution through the column, to filter. This process is one of the simplest methods used in research, although unfortunately, the activity of the enzyme can sometimes be compromised to a certain extent (not fully compromised).

You may try Biovision's Catalogue# K377 - Thrombin Cleavage Kit, which removes affinity tags and it also comes with a positive control. However, please note that we have not tested Cat # K377 with Cat# 7701-5 and hence we do not know the exact efficiency. You can also buy any other commercially available kits/thrombin enzyme/columns of your choice to perform the cleavage reaction.