Akt/PKB Antibody

Catalog #: 3247 | abID: ab18785

Product Details

abID ab18785
Antibody Target Akt/PKB
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Synthetic peptide corresponding to residue surrounding amino acid 80 of human Akt
Accession # P31749
Gene ID 60391226
Appearance Colorless liquid
Concentration 0.5 mg/ml
Formulation In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin
Purification Affinity purified
Species Reactivity Human, mouse, rat, bovine
Application Western blot,Immunoprecipitation, Immunohistochemistry
Application & Usage Western blotting (0.5-4 µg/ml), in immunoprecipitation (10-40 µg/ml), and Immunohistochemistry (10-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Akt1, Akt2 and Akt3.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence sμggests that Akt functions to promote cell survival by actively inhibiting apoptosis.


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Artwohl M et al. (2007) Am. J. Physiol. Endocrinol. Metab. 293: E681-E689.
Saemann, M.D., et al. (2005) J. Clin. Invest. 115: 468-475.
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