Product Details
Cat # +Size | K435-40 |
---|---|
Size | 40 assays |
Detection Method | Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions |
Species Reactivity | Mammalian |
Applications | The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate. |
Features & Benefits | • Simple procedure • The kit is suitable for both research and drug discovery • The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody. |
Kit Components | • Kinase Extraction Buffer • Akt Specific Antibody • Protein A Sepharose • GSK-3α Protein/ATP Mixture • Kinase Assay Buffer • Phospho-GSK-3α Specific Antibody |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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