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AKR1C3 (Human) ELISA Kit

A Sandwich ELISA kit to quantitatively measure AKR1C3 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which is [are] known to the State of California to cause birth defects or other reproductive harm.  For more information go to  www.P65Warnings.ca.gov.
Catalog #: E4927
$695.00

Product Details

Cat # +Size E4927-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of AKR1C3 in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Recovery range: 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• This Sandwich ELISA is highly sensitive and highly specific for the detection of AKR1C3 in human samples. There is no significant cross-reactivity or interference between AKR1C3 and analogues
• Detection range: 0.156 - 10 ng/ml
• Sensitivity: 0.094 ng/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (10 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Evolocumab is a human IgG2 monoclonal antibody that targets proprotein convertase subtilisin/kexin type 9 (PCSK9). PCSK9 is a serine protease that binds to LDL receptors on the hepatocyte cell surface and promotes the lysosome-mediated degradation of these receptors. This results in decreased uptake and clearance of LDL-C (bad cholesterol) by the receptors. The monoclonal antibody binds to PCSK9 and prevents interaction with the LDL receptor. This promotes the recycling of the internalized LDL receptor to the hepatocyte cell surface and increased removal of LDL-C from the blood. BioSim™ anti-Evolocumab ELISA kit qualitatively measures antibodies to Evolocumab in human serum or plasma samples. The kit is based on the Sandwich ELISA principle. Standards and samples (serum or plasma) are added to the microtiter plate coated with Evolocumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to the antibodies to Evolocumab that is captured by Evolocumab coated on the wells. Following incubation, wells are washed and the enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies to Evolocumab in the sample or standard. The qualitative test results can be evaluated using cut-off values.


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