Product Details
| abID | ab204694 |
|---|---|
| Cat # +Size | K326-100 |
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 392/482 nm) |
| Applications | Screening/characterizing/studying AHCY inhibitors |
| Features & Benefits | • Simple and reliable test to screen AHCY inhibitors • High-throughput suitable • Includes Inhibitor Control (3-Deazaneplanocin A) |
| Kit Components | • AHCY Assay Buffer • AHCY Reconstitution Buffer • AHCY Enzyme • Adenosine Deaminase • 3-Deazaneplanocin A (10 µM) (in DMSO) • AHCY Substrate (in DMSO) • Thiol Detecting Reagent (in DMSO) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies). In BioVision’s AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.
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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
