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Advance™ DNA Polymerase

Taq DNA Polymerase
Catalog #: M1151
SKU-Size Size Price Qty
M1151-250 250 U (50 ul)
$240.00
M1151-1000 1000 U (200 ul)
$640.00
More Sizes Get Quote

Product Details

Highlights Taq DNA Polymerase
Storage Conditions -20°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Taq DNA Polymerase


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Can I vortex the reagents?
Yes, the reagents can be vortexed.
What is the Mg2+ concentration in the 10X PCR buffer?
The concentration of Mg2+ in the 10X PCR buffer provided is 15 mM. The final concentration of Mg2+ in the reaction is 1.5 mM.
How can I optimize the PCR reaction for multiplexing?
The concentrations of DNA Polymerase, Mg2+ and the primers have to be optimized for each amplicon in multiplexing. The concentrations will need to be determined by the end user. The general rule would be to lower the concentrations of the primers for strongly amplified targets and increase the concentrations of the primers for weakly amplified targets.
Is Advance™ DNA Polymerase (BV Cat# M1151) compatible with Uracil?
Advance™ DNA Polymerase cannot incorporate dUTP and read through uracil present in DNA templates.
Can I use Advance™ DNA Polymerase (BV Cat# M1151) for A/T and G/C rich templates?
Yes, Advance™ DNA Polymerase (BV Cat# M1151) can be used for A/T and G/C rich templates. Please refer to the table on DNA Polymerases in the link
https://www.biovision.com/products/molecular-biology-tools/pcr-rtpcr-qpcr-dna-polymerases/dna-polymerases.html?dir=asc&order=sku
What is the source of the enzyme?
M1151 is a recombinant enzyme expressed in E.coli.