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ADPsensor™ Universal Kinase Activity Assay Kit

Fast, sensitive, High-throughput compatible, fluorometric Kinase assay with detection limit as low as 1 ng of active Kinase.
Catalog #: K212

Product Details

Cat # +Size K212-100
Size 100 assays
Detection Method Fluorometric (Ex/Em = 535/587 nm)
Species Reactivity Universal
Applications Detection of Kinase activity, Characterization of Kinase substrates and inhibitors.
Features & Benefits • Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement.
• Can detect as low as 1 ng of active Kinase.
• Free of ATP interference (up to 1 mM)
Kit Components • Kinase Assay Buffer
• ADP Sensor I
• ADP Sensor II
• Probe (in DMSO)
• Ultra-pure ATP (ADP depleted)
• DTT (1M)
• ADP Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Protein Kinases belong to a large family of phosphotransferases that mediate a wide spectrum of metabolic events via signal transduction pathways. Protein Kinases catalyze transfer of a phosphate group from a phosphate donor to a substrate protein. Kinases modulate the function of numerous proteins and are well-known therapeutic targets in many diseases like cancer, inflammation and diabetes etc. BioVision’s ADPsensor™ Universal Kinase Assay Kit is based on the fluorescence detection of ADP generated by Kinase activity. In this assay, ADP formed during the kinase reaction is converted to an intermediate by the ADP Sensor Mix, which then reacts with a probe to generate a strong and stable fluorescence signal at Ex/Em = 535/587 nm. ATP in the kinase reactions doesn’t interfere with the assay; therefore our kinase assay can be read kinetically (or at endpoint). The kit provides a universal tool to measure kinase activity in presence of substrates of your interest, or to screen/characterize kinase inhibitors. The assay is simple, non-radioactive, highly sensitive (<0.05 µM ADP) with broad ATP tolerance (up to 1 mM). The kit can detect as low as 1 ng of active Kinase.

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Ill residual ATP in sample effect the read? How does the kit deal with AMP formation?
1. This kit is designed to exclusively detect ADP formation.
2. Residual ATP in sample will not effect the read.
3. The ADP-dependent, enzyme-coupled reaction is much faster compared to AMP formation.
How to prepare sample from crude cells?
Start with ~2X106 cells, suspend the cell pellet 500 µl (or ~4 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the microscope. You can also use a sonicator, but sonicate for a few seconds only on ice. Use the eluate for your subsequent assays. Appropriate dilutions of the sample must be tested in order ensure the readings will fall within the linear range of the standard curve.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.