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Mag-Adenovirus Purification Kit

Magnetic beads based kits
Catalog #: K1459
SKU-Size Size Price Qty
K1459-20 20 preps
K1459-100 100 preps
More Sizes Get Quote

Product Details

Detection Method Magnetic beads based virus purification
Species Reactivity All
Applications PCR, western blot, ELISA, transfection directly with viruses bound to magnetic beads or eluted from the magnetic beads (free of beads).
Features & Benefits • Ideal for cell culture transduction/infection
• High yield and fast concentration (2 to 1000X)
• Purify viruses in 30-45 min
• Does not need ultracentrifugation
Kit Components • AD-Magnetic Beads
• Conservation Buffer (5X)
• Elution Buffer (1X)
Storage Conditions 4°C
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s Mag-Adenovirus Purification Kit is used to capture, concentrate and store adenoviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally viruses are purified by ultracentrifugation using CsCl which is time consuming and limited to the volume of cell lysate to be processed. BioVision’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Adenovirus (AD)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications. The AD-magnetic beads can be combined with any adenoviruses such as Ad Type 5 (Ad5), Oncolytic Ad (Ad520).

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Do we need to lyse the cells before adding the AD-magnetic beads in order to free the viral particles produced inside the cells and to elevate the viral titer in the supernatant?
This could be an option, but we do not recommend it. Complex formation between viral particles and magnetic nanoparticles is based on the hydrophobic and electrostatic interaction. It is not antibody mediated and thus cell lysate could interfere with the proper binding. Moreover, lysed cells may release viral particles including totally complete and active ones and also not fully finished non-infectious ones. This could impair the overall multiplicity of infection (MOI) or the overall immune response. For example, for the same MOI, total viral particle would be higher. Thus, we recommend (1) to capture viral particles in the supernatant and (2) try increasing the number by lysing the cells after the particles are removed from the supernatant. That way, the overall number could be raised but the MOI would not be impaired with cellular particles.
Do the magnetic beads provided in the kit show specificity for the virus? What is the principle behind the specific binding of beads to Adenovirus (AD) or the specific binding to Lentivirus (LV) or Retrovirus (RV)?
There is no specificity for the virus. Binding occurs through electrostatic and hydrophobic interactions between viruses and the beads surface. Our magnetic beads are positively charged and interact with viruses, which are negatively charged. The kit does not use antibodies directed against the specific viral epitope to capture the viral particle. They are thus mainly ubiquitous for any viral particles. Their main difference resides in the capacity of each magnetic bead to preferentially bind enveloped (LV or RV-magnetic beads) or non-enveloped (AD-magnetic beads) viral particles.
Is there a limit in the maximum number of viral particles that can be purified using BV Cat# K1458 and K1459?
It is difficult to precisely know the limit of the maximal number of viral particles that can be captured with one kit. This depends on many factors such as medium composition, serotype, final volume, salt balance etc.

For eg. In theory and as mentioned in the protocol, 20 µL of LV/RV or AD-magnetic beads are sufficient to bind 1x10^6 viral particles. Thus, 1 ml kit should be able to bind up to 5x10^7 viral particles. However, this is only theoretical as we generally speak in terms of active & infectious particles

We actually measure the infectivity efficiency of viruses (and not the whole population of viral particles). Based on the literature depending the virus, there could be a factor of >1000 between number of active particles and total particles. Thus, depending on the type of virus and the serotype, total number of viral particles that can be captured using 1 ml kit could be raised to at least to 10^10. Moreover, as there is no antibody or ligand-induced binding, the chances of saturation due to steric hindrance are very low.