Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

Only kit on the market to measure AHCY activity
Catalog #: K807 | abID: ab197002

Product Details

abID ab197002
Cat # +Size K807-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Applications Detection of AHCY activity
Features & Benefits • Rapid, simple & convenient • The assay can detect AHCY activity as low as 1 µU recombinant human AHCY.
Kit Components • AHCY Assay Buffer
• Homogenization Buffer
• OxiRed™ Probe
• SAH Substrate
• Enzyme Mix I
• Enzyme Mix II
• Adenosine Standard (10 mM)
• AHCY Enzyme (Positive Control)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Adenosylhomocysteinase (AHCY) (EC or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. BioVision’s AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed™ Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant human AHCY.

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