Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Adenosine Deaminase (ADA1) Inhibitor Screening Kit (Colorimetric)

Rapid & simple assay to screen adenosine deaminase potential inhibitors
Catalog #: K993
$495.00

Product Details

Cat # +Size K993-100
Size 100 assays
Detection Method Absorbance (OD 293 nm)
Species Reactivity Human
Applications Screening of potential Adenosine Deaminase inhibitors
Features & Benefits • Rapid, and convenient
• The kit screens potential human Adenosine Deaminase Inhibitors
Kit Components • ADA Assay Buffer (10X)
• ADA Convertor
• ADA Developer
• ADA Substrate
• ADA Enzyme
• ADA Inhbitor (EHNA)
• U.V. transparent plate (96-well)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Adenosine Deaminase (ADA, E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2’-deoxyadenosine to inosine and 2’-deoxyinosine. Adenosine is a metabolite that plays important roles in determining cell proliferation, cell cycle progression, and immunosuppressive activation. Although multiple pathways exist for the production of adenosine, its deamination is irreversible, and becomes a critical point in purine metabolite signaling. Both adenosine and inosine bind the adenosine A2A receptor yet can trigger different downstream signaling. Excess adenosine in penile tissue contributes to the disorder called priapism, and impaired adenosine signaling is associated with erectile dysfunction. For this reason, substantial work has gone into identification of inhibitors that would be of clinical relevance. The major isoform of adenosine deaminase, ADA1, is widely expressed in most cells in humans, particularly in lymphocytes and macrophages. ADA1 is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane while ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Adenosine signaling is part of the immune response, and has been shown to reduce T-cell activation. Adenosine Deaminase activity is thus a marker for T-lymphocyte proliferation. BioVision’s Adenosine Deaminase (ADA1) Inhibitor Screening Kit includes the larger 41 kDa form, human ADA1. In BioVision’s ADA inhibitor screening kit, adenosine is deaminated by ADA1 forming inosine. This intermediate is converted to uric acid by a series of enzymatic reactions. The formed uric acid can be measured in the supplied U.V. transparent plate at OD 293 nm. A highly specific ADA1 inhibitor is included as a positive control for inhibition. The kit provides a rapid, simple, sensitive, and reliable test, suitable for high-throughput screening of ADA1 inhibitors.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.