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Adenosine Deaminase Activity Assay Kit (Fluorometric)

Rapid & simple assay to measures total adenosine deaminase activity
Catalog #: K328

In stock

$345.00

Product Details

Cat # +Size K328-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Applications Detection of Adenosine Deaminase activity
Features & Benefits • Rapid, and convenient
• The kit measures total Activity of Adenosine Deaminase with limit of quantification 10 µU recombinant Adenosine Deaminase. Range of the assay: 10-100 µU.
Kit Components • ADA Assay Buffer (10x)
• ADA Convertor
• ADA Developer
• ADA Substrate
• ADA Probe
• ADA Positive Control
• Inosine Standard (10 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2’-deoxyadenosine to inosine and 2’-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In BioVision’s ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.


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What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.