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Product Details
| Cat # +Size | K327-100 |
|---|---|
| Size | 100 reactions |
| Detection Method | Fluorescence (Ex/Em = 535/587 nm) |
| Applications | Measurement of Adenosine in plasma and urine |
| Features & Benefits | • Rapid & convenient • Detection range: 2-80 pmol |
| Kit Components | • Adenosine Assay Buffer • Urine Clarifier • Adenosine Detector • Adenosine Convertor • Adenosine Developer • Adenosine Standard (10 mM) • Adenosine Probe (DMSO) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Adenosine, a purine nucleoside, is present throughout the body. It plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and also regulates the Renin-Angiotensin system. It also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure. In BioVision’s Adenosine Assay, adenosine is measured using adenosine deaminase followed by a multi-step enzymatic approach resulting in the generation of an intermediate that reacts with the Adenosine Probe with the formation of a fluorescent product. The fluorescent product is measured at Ex/Em = 535/587 nm. Detection range: 2-80 pmol.
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Can ATP interfere with the assay?
The kit has a very specific converter system that helps in assaying for adenosine specifically as opposed to ATP, AMP and ADP.
Do you know what reagents can interfere with the assay?
Inosine, xanthine and hypoxanthine present in the sample(s) will contribute to the background. PBS, DMEM or RPMI should not contribute background as long as they do not contain supplemented inosine, xanthine/hypoxanthine.
Is it possible to use your adenosine kit to measure adenosine in a tissue sample?
We have not used this kit for tissue adenosine quantification. There might not be a large amount in this sample type to begin with, so you might need to optimize the sample amount needed. Homogenize tissues in the assay buffer in the kit and centrifuge to get a tissue lysate. Use this for the assay.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Bimal, Sanjiva et al. (2017) Adenosine generated by ectonucleotidases modulates the host immune system during visceral leishmaniasis, Cytokine. 2017 Mar;91:170-179.
For more citations of this product click here
