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ADAM33 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of ADAM33

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5102
$750.00

Product Details

Cat # +Size E5102-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of ADAM33 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Sensitivity: 46.875 pg/ml
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Highly sensitive and specific
● Recovery Rate: 87-102% for serum, 89-102% for EDTA plasma and 90-105% for heparin plasma
● Detection range: 78.125-5000 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

ADAM33 is a member of the ADAM (a disintegrin and metalloproteinase) gene family and have been implicated in a variety of biological processes involving cell–cell and cell–matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms. The preferential expression of ADAM33 in mesenchymal cells and its multiple molecular actions provide ample opportunity for incriminating this molecule in chronic asthma. The identification of ADAM33 as a major risk factor involved in the pathogenesis of bronchial hyperresponsiveness and airway wall remodeling provides insight into the pathogenesis of asthma and represents a novel therapeutic target. ADAM33 also acts as a novel molecular biomarker of Triple-negative breast cancer and BLBC that might be useful as a prognostic factor. BioVision’s ADAM33 (Human) ELISA Kit is used for the quantitative detection of ADAM33 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ADAM33 in the sample.


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