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ADAM29 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of ADAM29

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5106
$750.00

Product Details

Cat # +Size E5106-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of ADAM29 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Recovery Rate: 86-103% for serum, 95-100% for EDTA plasma and 85-99% for heparin plasma
● Sensitivity: 0.094 ng/ml
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Highly sensitive and specific
● Detection range: 0.156-10 ng/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

ADAM29 is a member of the ADAM (a disintegrin and metalloproteinase) gene family which is involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. ADAM29 is a type I integral membrane protein and secrets a glycoprotein that mediates cell-cell and cell-matrix interaction. It is involved in a number of physiological processes diversification. ADAM29 is increased in gastric cancer and the elevated expression of ADAM29 is associated with a poor survival rate of patients. ADAM29 becomes a prognostic factor and therapeutic candidate for human gastric cancer. ADAM29 plays a certain role in the signal transduction during the maturation of testis-associated cells. BioVision’s ADAM29 (Human) ELISA Kit is used for the quantitative detection of ADAM29 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ADAM29 in the sample.


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