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ADAM12 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of ADAM12

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5036
$750.00

Product Details

Cat # +Size E5036-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of ADAM12 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Sensitivity: 0.094 ng/ml
● Recovery Rate: 92-105% for Serum, 89-102% for EDTA plasma and 89-104% for Heparin plasma
● Highly sensitive and specific
● Detection range: 0.156-10 ng/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Disintegrin and metalloproteinase domain-containing protein12 also known as ADAM12 belongs to the ADAM (a disintegrin and metalloprotease) protein family. ADAM12 is expressed in placenta, skeletal, cardiac, and smooth muscle. ADAM12 is involved in skeletal muscle regeneration, specifically at the onset of cell fusion and also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. They are also expressed in some tumor cells lines. Additionally, it binds insulin growth factor binding protein-3 (IGFBP-3) and appears to be an effective early Down syndrome marker. ADAM12 has also been implicated in the development of pathology in various cancers, hypertension, liver fibrogenesis and asthma. BioVision’s ADAM12 (Human) ELISA Kit is used for the quantitative detection of ADAM12 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ADAM12 in the sample.


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