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Acyl-CoA Synthetase Fluorometric Assay Kit

To detect ACS activity in various samples
Catalog #: K184
$500.00

Product Details

Cat # +Size K184-100
Size 100 assays
Kit Summary BioVision’s Acyl-CoA Synthetase Fluorometric assay kit provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/µl in various samples.
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity All
Applications Measurement of Acyl-CoA Synthetase activity in various tissues/cells
Features & Benefits • Rapid
• Sensitive
• Straightforward way to measure ACS activity in various samples
Kit Components • ACS Enzyme Mix
• H₂O₂ Standard
• ACS Substrate
• ACS Assay Buffer
• ACS Probe
• ACS Developer
• ACS Converter
• ACS Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Acyl-CoA Synthetase (ACS) also known as Acyl-CoA ligase, is a ubiquitous enzyme that catalyzes the reaction of free fatty acids and Coenzyme A (CoA) to generate Fatty Acyl-CoAs through an ATP-dependent mechanism. The incorporation of this CoA moiety generates a thio-ester bond that effectively activates the fatty acid for downstream biological processes. Unlike their fatty acid counterparts, Fatty acyl-CoAs also serve as precursors of cellular lipids. Dysregulation of the various isoforms of ACS have been implicated in a number of metabolic diseases, including Non-Alcoholic Fatty Liver Disease (NAFLD) and Hepatic Fibrosis. ACS also plays a role in regulating apoptosis. BioVision’s Acyl-CoA Synthetase Fluorometric assay kit provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/µl in samples.


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