Acyl-CoA Synthetase Assay Kit (Fluorometric) (ab273315)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Adherent cells, Purified protein, Suspension cells, Tissue Extracts, Tissue sections
Overview
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Product name
Acyl-CoA Synthetase Assay Kit (Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Adherent cells, Suspension cells, Tissue Extracts, Purified protein, Tissue sections -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Acyl-CoA Synthetase (ACS) Assay Kit (Fluorometric) (ab273315) provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/µl sample.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K184 Acyl-CoA Synthetase Fluorometric Assay Kit. K184-100 is the same size as the 100 test size of ab273315.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ACS Assay Buffer 1 x 25ml ACS Converter (Lyophilized) 1 vial ACS Developer (Lyophilized) 1 vial ACS Enzyme Mix (Lyophilized) 1 vial ACS Positive Control (Lyophilized) 1 vial ACS Probe 1 x 0.2ml ACS Substrate 1 x 1.1ml H2O2 Standard 1 x 100µl
Images
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Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Reaction kinetics of ACS Positive Control.
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ACS activity in Rat liver lysate. Rat liver was prepared according to the described protocol. For activity determination, experiments were run in duplicates. 3.3-13.3 µg protein was loaded per well.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273315 has not yet been referenced specifically in any publications.