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Active Sonic Hedgehog (SHH), murine recombinant

Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
Catalog #: 4020
SKU-Size Size Price Qty
4020-25 25 μg
$255.00
4020-100 100 μg
$595.00
4020-1000 1 mg
$3,835.00
More Sizes Get Quote

Product Details

Alternate Name SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Gene Symbol SHH
Gene ID 20423
Accession # Q8C765
Source E. coli
Appearance Lyophilized protein
Physical Form Description Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
Molecular Weight 19.8 kDa
Purity by SDS-PAGE ≥97%
Purity by ≥97%
Endotoxin Level <0.01 ng/μg
Biological Activity Mouse SHH is fully biologically active when compared to standard. Determined by it’s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED₅₀ in the range of 0.03 – 0.30 ug/ml.
Reconstitution Instructions When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which c
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da.


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