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Active Sonic Hedgehog (SHH), human recombinant

Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
Catalog #: 4010

In stock

SKU-Size Size Price Qty
4010-25 25 μg
$240.00
4010-100 100 μg
$775.00
4010-1000 1 mg
$3,835.00
More Sizes Get Quote

Product Details

Alternate Name SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Gene Symbol SHH
Gene ID 6469
Accession # Q15465
Source E. coli
Appearance Lyophilized protein
Physical Form Description Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
Molecular Weight 19.697kDa
Purity by SDS-PAGE ≥97%
Purity by ≥97%
Endotoxin Level <0.01 ng/μg
Biological Activity Human recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED₅₀, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml.
Reconstitution Instructions When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which c
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Recombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.


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