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Product Details
| Alternate Name | NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2. |
|---|---|
| Gene Symbol | SIRT2 |
| Gene ID | 22933 |
| Accession # | Q8IXJ6 |
| Source | E. coli. |
| Appearance | Sirtuin 2 (SIRT2) with a His-tag is supplied as lyophilized powder. |
| Physical Form Description | Human Sirtuin 2 is lyophilized from 50 mM Tris, pH 8.0. |
| Molecular Weight | Human SIRT2 is a 60 kDa (2 to 389 aa + NT His Tag) protein |
| Purity by SDS-PAGE | ≥90% |
| Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in 25 mM Tris, pH 7.5 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2 and 35% Glycerol to a concentration of 0.1 – 1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be stored at –20 °C for future use. For long term storage, it is recommended to add a carrier protein (0.1% BSA) in above reconstitution solution. Avoid multiple freeze/thaw cycles. |
| Specific Activity | ≥ 0.5 mU/mg |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
Sirtuins function as intracellular regulatory proteins. Human Sirtuin 2 is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. Human SIRT2 is a cytoplasmic protein responsible for the deacetylation of histone H4 and a-tubulin, a modification important for controlling the cell cycle. Specifically, SIRT2 protein co-localizes with HDAC6 and microtubules and functions as a mitotic checkpoint in preventing chromosomal instability that can lead to hyperploid cells. SIRT2 is found in many tissues, but is specifically enriched in skeletal muscle, the heart, and in oligodendroglia cells in the brain. The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (0.1mM NAD+) dependent and insensitive to HDAC inhibitor trichostatin A.
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