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Active AHCY, human recombinant

Catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy).
Catalog #: 7527
SKU-Size Size Price Qty
7527-10 10 μg
$195.00
7527-50 50 μg
$395.00
7527-1000 1 mg
$4,995.00
More Sizes Get Quote

Product Details

Alternate Name SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosyl Homocysteinase
Gene Symbol AHCY
Gene ID 191
Accession # P23526
Source E. coli
Appearance Liquid
Physical Form Description 5 mg/ml in 50 mM potassium phosphate, 1 mM EDTA, pH 7.2 containing 20% glycerol.
Molecular Weight 52.1 kDa (1-432 aa + N-terminal polyhistidine tag). In the native form the protein exists in the tetrameric form and this form is required for activity.
Purity by SDS-PAGE ≥90%
Biological Activity BioVision’s Active AHCY has been tested for its activity using Adenosyl Homocysteinase (AHCY) Activity Fluorometric Assay Kit (K807-100). It has a specific activity of >350 mU/mg.
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

AHCY (EC 3.3.1.1) is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AHCY controls the intracellular S-adenosylhomocysteine (SAH) concentration that is crucial for transmethylation reactions. AHCY deficiency causes hypermethioninemia. The recombinant human AHCY produced in E.Coli is a single, non-glycosylated polypeptide chain and is purified by proprietary chromatographic techniques.


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