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Acidic Mammalian Chitinase Inhibitor Screening Kit (Fluorometric)

Catalog #: K693
$525.00

Product Details

Cat # +Size K693-100
Size 100 assays
Detection Method Fluorescence (RFU= 320/445 nm)
Applications Screening/characterizing/studying Mammalian Chitinase inhibitors/activators
Features & Benefits • Simple and reliable test to screen AMCase inhibitors/activators
• High-throughput compatible
• Includes Inhibitor Control (Bisdionin F)
Kit Components • AMCase Assay Buffer• AMCase Substrate (in DMSO)• Acidic Mammalian Chitinase (lyophilized)• Bidionin F (5 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Acidic Mammalian Chitinase (AMCase) belongs to the glycosyl hydrolase-18 family (EC 3.2.1.14), which is capable of hydrolyzing the β-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. AMCase is relatively abundant in the gastrointestinal tract and lung. It is highly expressed in the lungs of asthmatic patients and asthma animal models. In addition, the change in AMCase expression can be used to monitor Th-2/IL-13 mediated immune response and inflammation. Inhibition of AMCase activity has been considered as a therapeutic strategy to alleviate symptoms of allergic airway inflammation. Thus, there is a need to identify AMCase inhibitors as potential candidates to treat and/or prevent asthma. Biovision’s Acidic Mammalian Chitinase Inhibitor Screening Kit uses Bisdionin F— a highly selective AMCase inhibitor as positive control and it provides a simple, rapid, sensitive and reliable test suitable for screening/characterizing AMCase inhibitors.


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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.