Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Acid Sphingomyelinase Assay Kit II (Colorimetric)

Complete kit that allows the measurement of Acid Sphingomyelinase in biological samples
Catalog #: K192
$375.00

Product Details

Cat # +Size K192-100
Size 100 assays
Detection Method Colorimetric (OD= 570 nm)
Species Reactivity All
Applications Measurement of ASMase activity in various tissues/cell extracts
Features & Benefits • Complete set of reagents for the detection of Acid SMase in biological samples
• Simple, specific (detect acid SMase activity only)
Kit Components • Choline Standard (Lyophilized)
• ASMase Assay Buffer I
• ASMase Enzyme Mix I (Lyophilized)
• ASMase Enzyme Mix II (Lyophilized)
• ASMase Probe (DMSO)
• ASMase Assay Buffer II
• ASMase Enhanceer
• ASMase Substrate (Lyophilized)
Storage Conditions -20°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyelinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
What does this kit detect - Acid Sphingomyelinase from lysosome, the secretory sphingomyelinase or both?
The kit detects both.