Acid Sphingomyelinase Assay Kit (Colorimetric) (ab252889)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Adherent cells, Serum, Suspension cells, Tissue
- Sensitivity: 3 mU/ml
Overview
-
Product name
Acid Sphingomyelinase Assay Kit (Colorimetric)
See all Acid sphingomyelinase kits -
Detection method
Colorimetric -
Sample type
Serum, Tissue, Adherent cells, Suspension cells -
Sensitivity
3 mU/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Species independent -
Product overview
Acid Sphingomyelinase Assay Kit (Colorimetric) ab252889 provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K192 Acid Sphingomyelinase Assay Kit II (Colorimetric). K192-100 is the same size as the 100 test size of ab252889.
-
Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ASMase Assay Buffer I 1 x 20ml ASMase Assay Buffer II 1 x 15ml ASMase Enhancer 1 x 100µl ASMase Enzyme Mix I (Lyophilized) 1 vial ASMase Enzyme Mix II (Lyophilized) 1 vial ASMase Probe (DMSO) 1 x 0.2ml ASMase Substrate (Lyophilized) 1 vial Choline Standard (Lyophilized) 1 vial -
Research areas
-
Function
Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. -
Involvement in disease
Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPDA) [MIM:257200]; also known as Niemann-Pick disease classical infantile form. It is an early-onset lysosomal storage disorder caused by failure to hydrolyze sphingomyelin to ceramide. It results in the accumulation of sphingomyelin and other metabolically related lipids in reticuloendothelial and other cell types throughout the body, leading to cell death. Niemann-Pick disease type A is a primarily neurodegenerative disorder characterized by onset within the first year of life, mental retardation, digestive disorders, failure to thrive, major hepatosplenomegaly, and severe neurologic symptoms. The severe neurological disorders and pulmonary infections lead to an early death, often around the age of four. Clinical features are variable. A phenotypic continuum exists between type A (basic neurovisceral) and type B (purely visceral) forms of Niemann-Pick disease, and the intermediate types encompass a cluster of variants combining clinical features of both types A and B.
Defects in SMPD1 are the cause of Niemann-Pick disease type B (NPDB) [MIM:607616]; also known as Niemann-Pick disease visceral form. It is a late-onset lysosomal storage disorder caused by failure to hydrolyze sphingomyelin to ceramide. It results in the accumulation of sphingomyelin and other metabolically related lipids in reticuloendothelial and other cell types throughout the body, leading to cell death. Clinical signs involve only visceral organs. The most constant sign is hepatosplenomegaly which can be associated with pulmonary symptoms. Patients remain free of neurologic manifestations. However, a phenotypic continuum exists between type A (basic neurovisceral) and type B (purely visceral) forms of Niemann-Pick disease, and the intermediate types encompass a cluster of variants combining clinical features of both types A and B. In Niemann-Pick disease type B, onset of the first symptoms occurs in early childhood and patients can survive into adulthood. -
Sequence similarities
Belongs to the acid sphingomyelinase family.
Contains 1 saposin B-type domain. -
Cellular localization
Lysosome. - Information by UniProt
-
Alternative names
- Acid sphingomyelinase
- ASM
- ASM_HUMAN
see all
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Functional Studies |
Use at an assay dependent concentration.
|
Notes |
---|
Functional Studies
Use at an assay dependent concentration. |
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab252889 has not yet been referenced specifically in any publications.