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Acid Sphingomyelinase Assay Kit II (Colorimetric)

Complete kit that allows the measurement of Acid Sphingomyelinase in biological samples
Catalog #: K192

Product Details

Cat # +Size K192-100
Size 100 assays
Detection Method Colorimetric (OD= 570 nm)
Species Reactivity All
Applications Measurement of ASMase activity in various tissues/cell extracts
Features & Benefits • Complete set of reagents for the detection of Acid SMase in biological samples
• Simple, specific (detect acid SMase activity only)
Kit Components • Choline Standard (Lyophilized)
• ASMase Assay Buffer I
• ASMase Enzyme Mix I (Lyophilized)
• ASMase Enzyme Mix II (Lyophilized)
• ASMase Probe (DMSO)
• ASMase Assay Buffer II
• ASMase Enhanceer
• ASMase Substrate (Lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyelinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

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What does this kit detect - Acid Sphingomyelinase from lysosome, the secretory sphingomyelinase or both?
The kit detects both.