Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Acetylcholinesterase Inhibitor Screening Kit (Colorimetric)

Highly Sensitive Assay
Catalog #: K197

Product Details

Cat # +Size K197-100
Size 100 assays
Kit Summary • Detection: Absorbance (OD 412 nm)
• Application:
- Screening/characterizing Acetylcholinesterase inhibitors
Detection Method Absorbance (OD 412 nm)
Applications • Screening of AChE inhibitors
Features & Benefits • Rapid, simple & convenient
• This assay kit can screen potential human acetylcholinesterase inhibitors
Kit Components • AChE Assay Buffer
• AChE Substrate
• Acetylcholineterase
• Probe Mix
• Donepezil (10 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Acetylcholinesterase (AChE; EC is the main cholinesterase enzyme in the nervous system. It is responsible for the hydrolysis of acetylcholine and some other choline esters that function as neurotransmitters. AChE is highly expressed in both the brain and the peripheral nervous system. It is especially enriched at neuromuscular junctions, where its activity serves to terminate synaptic transmission between motor neurons and skeletal muscles. Reversible inhibitors of AChE (such as donepezil, rivastigmine and galantamine) are commonly used in the treatment of Alzheimer’s Disease and other neurodegenerative disorders. However, irreversible inhibitors of AChE tend to be extremely poisonous, causing muscular paralysis, convulsions, bronchial constriction and eventual death by asphyxia. BioVision’s Acetylcholinesterase Inhibitor Screening Kit can be used to screen for potential inhibitors of AChE activity. It utilizes the ability of an active human AChE enzyme to hydrolyze the provided colorimetric substrate, generating a yellow chromophore that can be detected by measuring absorbance at 412 nm. In the presence of the potent reversible AChE inhibitor donepezil, enzyme activity is suppressed, preventing chromophore generation. The assay kit is adapted to a 96-well format and provides a rapid, simple and reliable test for high-throughput screening of AChE inhibitors

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.