A Sandwich ELISA Kit for quantitative measurement of Anti-Cyclic Citrullinated Peptide Antibody (ACCPA) in human serum, plasma, tissue lysates, and other biological fluids

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Catalog #: E4965 | abID:

Product Details

Cat # +Size E4965-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA Kit to quantitatively measure ACCPA in human serum, plasma, tissue lysates and other biological fluids
Features & Benefits ● Detection range: 1.563 – 100 ng/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● This Sandwich ELISA is highly sensitive and highly specific for the detection of ACCPA in human samples. There is no significant cross-reactivity or interference between ACCPA and analogues
● Sensitivity: 0.938 ng/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (100 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Anti-cyclic citrullinated peptide antibodies (ACCPA) are autoantibodies that target citrullinated peptides or proteins. Citrullination or deimination is a posttranslational modification wherein peptidyl arginine is converted to citrulline by peptidyl arginine deiminases (PADIs). Citrullination of proteins is observed in inflammatory diseases such as Alzheimer's disease, multiple sclerosis, rheumatoid arthritis, and cancers. In joints, the citrullination of proteins triggers an immune response that results in the formation of autoantibodies such as ACCPA against joint proteins. ACCPA constitutes about 60-80% of the serum of patients with rheumatoid arthritis (RA) and can help diagnose RA at an early stage. Hence, ACCPA is considered a diagnostic biomarker of rheumatoid arthritis. BioVision’s ACCPA (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of ACCPA in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of ACCPA in the sample or standard.

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