ABTS™ Solution

A peroxidase substrate

1212 is available from Abcam as ab142041.
Catalog #: 1212 | abID: ab142041

Product Details

abID ab142041
Cat # +Size 1212-100
Size 100 ml
Alternate Name 2,2'-Azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid)
Appearance Liquid
Formulation 1.46 mMol L⁻¹, in a citrate buffer, pH 4.0.
CAS # 28752-68-3
Molecular Formula C₁₈H₁₈N₄O₆S₄
Molecular Weight 514.6
SMILES CCN1C2=C(C=C(C=C2)S(=O)(=O)O)SC1=NN=C3N(C4=C(S3)C=C(C=C4)S(=O)(=O)O)CC
InChi InChI=1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)
PubChem CID 35688
MDL Number MFCD00214141
Handling Protect from light
Storage Conditions 2-8 °C
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.


ABTS is considered a safe and sensitive substrate compatible for horseradish peroxidase (HRP) based ELISA assays. In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation with an absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405 nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength. Using a proprietary-nontoxic stabilization technology, BioVision, Inc. provides a room temperature stable, single component ABTS solution for quantitative analysis of HRP based systems.

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