ABTS™ (solid)

A peroxidase substrate
Catalog #: B2711 | abID:

Product Details

Alternate Name 2,2'-(1,2-hydrazinediylidene)bis[3-ethyl-2,3-dihydro-6-benzothiazolesulfonic acid, diammonium salt
Peptide Sequence N/A
Appearance Light green powder
CAS # 30931-67-0
Molecular Formula C₁₈H₂₄N₆O₆S₄
Molecular Weight 548.68
Purity ≥98% by HPLC
Solubility ~10 mg/ml (DMSO); >40 mg/ml (Water)
SMILES [O-]S(C1=CC=C(N(CC)/C(S2)=N/N=C3SC(C=C(S([O-])(=O)=O)C=C4)=C4N\3CC)C2=C1)(=O)=O.[NH4+].[NH4+]
InChi InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
PubChem CID 9570474
MDL Number MFCD00010404
Handling Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


ABTS is considered a safe and sensitive substrate compatible for horseradish peroxidase (HRP) based ELISA assays. In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation with an absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405 nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength.

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