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8-epi-PGF2α ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4789
$545.00

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.
Features & Benefits • Sensitivity: 9.38 pg/mL
• Specificity: This kit recognizes 8-epi-PGF2α in samples. No Significant cross-reactivity or interference between 8-epiPGF2α and analogues was observed.
• Precision: Coefficient of variation is < 10%.
• Detection Range: 15.63—1000 pg/mL
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

F2‐isoprostanes are a family of prostaglandin F2‐like compounds that are formed by free‐radical‐catalyzed peroxidation of arachidonic acid. Several F2‐isoprostanes, but in particular 8‐epi PGF2α, are widely used as oxidative stress biomarkers. High levels of F2-IPs can disrupt the physicochemical integrity of cell membranes but more importantly can exert significant pharmacological activity. 8-epi-PGF2α is a potent vasoconstrictor acting on the renal, pulmonary, and retinal vasculatures, modulates platelet aggregation and is involved in the activation of intracellular signaling. BioVision 8-epi-PGF2α ELISA Kit is based on the Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2αin the samples or standard competes with 8-epi-PGF2α coated on the plate for binding to the anti-8-epi-PGF2αantibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microplate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of 8-epi-PGF2α. The concentration of 8-epi-PGF2αin the samples can be calculated by comparing the OD of the samples to the standard curve.


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