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6-keto-PGF1 α ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of 6-keto-PGF1α concentrations in serum, plasma and other biological fluids.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4790
$545.00

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in vitro quantitative determination of 6-keto-PGF1α concentrations in serum, plasma and other biological fluids.
Features & Benefits • Sensitivity: 9.38 pg/mL
• Precision: Coefficient of variation is < 10%.
• Detection Range: 15.63-1000 pg/mL
• Specificity: This kit recognizes 6-keto-PGF1a in samples. No Significant cross-reactivity or interference between 6-keto-PGF1a and analogues was observed.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

6-keto-PGF1α is a stable hydrolysis product of prostacyclin. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia. BioVision 6-keto-PGF1α ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with 6-keto-PGF1α. During the reaction, 6-keto-PGF1αin the samples or standard competes with 6-keto-PGF1α coated on the plate for binding to the anti-6-keto-PGF1αantibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of 6-keto-PGF1α. The concentration of 6-keto-PGF1αin the samples can be calculated by comparing the OD of the samples to the standard curve.


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