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5-hmC polyclonal antibody (rabbit)

Polyclonal Antibody to detect 5-hmC in human samples.
Catalog #: 6830

In stock

$245.00

Product Details

Cat # +Size 6830-50
Size 50 μl
Antibody Target 5-hmC
Alternate Name 5-hydroxymethylcytosine
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
Accession # N/A
Gene ID N/A
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Purification Whole Anti-Sera
Species Reactivity Human, mouse, wide range.
Application ELISA, hmEdIP, Dot blot.
Positive Control ELISA: Peptide, hmEdIP, Dot blot: Peptides.
Application & Usage hMeDIP: 2.5 µg/IP, Dot Blot: 1:200, ELISA: 1:500.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0.6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is abundant in brain tissue, especially in areas that are associated with higher cognitive functions. Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique rabbit polyclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.


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