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3T3-L1 Differentiation Kit

Catalog #: K579

In stock

$245.00

Product Details

Cat # +Size K579-100
Size For 100 ml differentiation medium
Applications Differentiation of 3T3-L1 preadipocytes to adipocytes
Study of obesity, adipogenesis, lipolysis and lipid metabolism
Features & Benefits • Simple, rapid & convenient method to differentiate 3T3-L1 preadipocytes to adipocytes
Kit Components • Insulin (1.5 mg/ml)
• Differentiation Cocktail 1000x (Lyophilized)
• DMSO (anhydrous)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. BioVision’s 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 µg/ml insulin, 1 µM dexamethasone, 500 µM IBMX, and 1 µM rosiglitazone in the differentiation media.


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What is the biological source of the insulin?
This is a human recombinant insulin isolated from e.coli
How to quantify adipogenesis?
For a protocol and chemical induction of differentiation in 3T3 cells you can use the protocol on the datasheet attached. Once the cells are differentiated, you can either histochemically look at the lipid droplets using this: http://www.biovision.com/lipid-oil-red-o-staining-kit-8056.html or use the K610 kit for colorimetric/fluorometric assay: http://www.biovision.com/adipogenesis-colorimetric-fluorometric-assay-kit-3726.html.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
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