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3D Cell Culture Ready-to-Use Scaffold Complete Kit

Catalog #: K990

Product Details

Cat # +Size K990-100
Size 100 assays
Detection Method Qualitative
Species Reactivity Mammalian
Applications • Spheroid Formation Assays
Features & Benefits Reproducible, Qualitative tool for screening, studying, and characterizing compounds in cell culture studies
Kit Components • Scaffold Dissociation Solution (enzyme-free) • Neutralization Buffer
• Ready-to-Use Scaffolds (96-well plate)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell culture environments improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell culture experiments. 3D matrices and scaffolds provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. BioVision offers 3D cell culture kits, including Basement Membrane Matrix (BME, animal-based), Alginate Hydrogel (plant-based), proprietary BioVision’s Duo-Matrix and dry Scaffold (natural polymers, animal-free) to meet the needs and requirements of various research fields. BioVision’s 3D Cell Culture Ready-to-Use Scaffold Complete Kit provides a standardized, and adaptable to high-throughput strategy microplate containing proprietary 3D scaffolds for setting up spheroid formations, 3D cell cultures, pharmacological studies, with an optimized scaffold dissociation method for subsequent biochemical or protein analysis

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Is it possible to grow primary keratinocytes on them to form a 3D skin-like structure?
K990-100 is meant for adherent cells and not primary cells. We have not tested primary cells with this kit.
Do you think the scaffold can be used to culture cells for long periods of time? If so, is a month ok?
Adherent cells have been cultured in scaffold for 21 days and have successfully formed spheroids. We have not tested for more than 21 days.
Can the cells be harvested for downstream applications?
Yes, please see Step VII-3 of the data sheet. Cells can be harvested and used for subsequent analysis.
What is the scaffold provided in the kit made of?
The ready-to-use scaffold is made of a natural polysaccharide found in brown algae.