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3’ to 5’ Exonuclease Activity Assay (Fluorometric)

For monitoring 3’ exonuclease activity in a wide variety of samples
Catalog #: K175

In stock

$495.00

Product Details

Cat # +Size K175-100
Size 100 assays
Kit Summary BioVision’s 3’ to 5’ Exonuclease Activity Assay Kit provides a quick and easy method for monitoring 3’ exonuclease activities in a wide variety of samples.
Detection Method Fluorescence (Ex/Em = 304/369 nm)
Species Reactivity All
Applications • Quality control of purified 3’ to 5’ exonuclease
• Measurement of 3’ to 5’ exonuclease activity in biological samples
Features & Benefits • Sensitive
• High-throughput adaptable
• Detection limit as low as 0.2 µU of exonuclease activity
• Simple
Kit Components • Exonuclease Probe
• Fluorescence Standard (2 mM in DMSO)
• Half-area Plate
• Exonuclease Assay Buffer
• Exonuclease Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Exonucleases remove a single nucleotide or an oligonucleotide at the 5’ or 3’ end. Exonuclease, such as Three Prime Repair Exonuclease 1 (TREX1), plays an important role in proofreading of DNA amplification and removal of damaged DNA. Deficiency of TREX1 enzyme has been shown to trigger autoimmune disease, Aicardia-Goutieres syndrome. TREX1 expression has been reported to correlate with cervical cancer cell growth and disease progression. In addition, 3’ exonucleases are often used as tools in molecular biology such as site-directed mutagenesis and the generation of single stranded DNA probe. BioVision’s 3’ to 5’ Exonuclease Activity Assay Kit provides a quick and easy method for monitoring 3’ exonuclease activities in a wide variety of samples. In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em= 304/369nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 µU of exonuclease activity.


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